GPCR cDNA Bank (View)

• High Quality:	All Multispan’s GPCR cDNA inserts have been full-length sequenced.
• High Expression:	All cDNAs have been cloned into proprietary vector for optimal GPCR expression in mammalian cells.
• Easy Detection:	All cDNAs are tagged at 5’ for monitoring GPCR protein expression.

GPCR cell lines for functional assays (View)

Multispan has generated high quality cell lines for GPCR high throughput drug screening assays. All the cell lines have been optimized for S/N ratio in Ca++ or cAMP responses and shown stability for at least 30 passages.

HEx^TM High expression cell lines (View)

In addition to stable cell lines expressing variety of GPCRs, we are proud to introduce cell lines expressing high quantities of GPCRs on the surface. These cell lines express up to 2x10⁶ molecules per cell that are fully functional as evidenced by normal Ca++ influx. These cell lines thus present a unique source of high quality GPCR proteins for variety of applications, including:
• Antibody generation using antigens with natural epitopes at high expression level
• Cell-based or membrane receptor binding assays to achieve high signal-to-noise ratio
• Cell-based functional assays for hard-to-express GPCRs
• Structural studies as high quality source of protein for crystallization