Multispan Inc.

1) GPCR monoclonal antibody development

Multispan uses a unique strategy to develop and deliver high-quality monoclonal antibodies against native GPCR epitopes.

Platform highlights:
• High quality antigen: >100,000 GPCR molecules expressed on per mammalian cell as native immunogen
• Against natural GPCR epitopes
• High specificity

2) GPCR assay development

• Functional cell-based assays:
Multispan has demonstrated proven track record in developing functional cell based assays for high-throughput screening using stably GPCR-transfected mammalian cell lines with optimal EC50 readouts for Ca++, cAMP or NFAT and CRE driven reporters.

• Membrane binding assays:
Multispan provides high quality membrane preparations with demonstrated ligand binding function and optimal EC50.

3) GPCR Deorphanization

GPCRs constitute the best pharmaceutical target family. Among them some 100 exist for which their natural ligand is unknown. These "orphan" GPCRs have the potential to serve as targets for novel drugs and novel indications. Yet to be used in pharmaceutical research, these GPCRs need first to be deorphanized, i.e. their natural ligand need to be discovered to allow for development of high-throughput screening assays. With Proven expertise and 10+ years of experience, Multispan provides deorphanization of orphan GPCRs as a service. Part of it will include establishment of high-throughput assays.

Multispan welcomes new contract research partnerships in the above areas. Please contact us at info@multispaninc.com or 510.887.0817, ext 101 for more information.

4) GPCR Compound Profiling

Our compound profiling services reduce time and costs of drug development. If you have a panel of lead candidates and need to concentrate on optimizing just some of them, Multispan can help you to make the right decision. We offer compound profiling services based on our fast growing collection of stable cell lines expressing different classes of GPCR. This collection serves as a source of representative targets that can be used to survey drug candidate specificity in functional assays like Ca++ influx.

We could also evaluate your drug candidate in membrane assays. Our high expression technology provides you with membranes that give the best Signal-to-Noise ratio for reliable results. This two-prong approach allows our clients to obtain valuable functional, biochemical, and physico-chemical information about drug candidates.

All intellectual property resulted from screening is retained by the client.