Multispan Inc.

Issue: There are 350 "druggable" GPCRs which share many similar primary and conformational epitopes. Is your antibody lead SPECIFIC to your GPCR target of interest?

Solution: Assess specificity of the antibody lead in Multispan's large GPCR expression panel. We measure your antibody binding to GPCRs in NATIVE conformations directly on mammalian cell surface by FACS.

Benefits: Obtain valuable information to
-make Go-Or-No-Go decision early
-save cost in expensive animal and clinical studies
-shorten lead development cycle

Convenience: Send us your purified, un-labeled antibody and you will have the results back in ONE week.

Flexibility: Choose your own panel from our 300 fully-sequenced full-length cDNAs already cloned in Multispan's powerful proprietary expression vector or use our fixed panel of 60 GPCRs below.

Validated Expression Panel of 60 GPCRs for Antibody Profiling
Anaphylatoxin	Galanin	Melanin-Concentrating H.	Purinergic
C3a	GAL1	MCH1	P2Y2
C5a	GAL2	Melanocortin	P2Y8
Apelin	Glucagon	MC4	P2Y10
APJ	glucagon	Muscarinic	P2Y12
Bombesin	GPRC5	M1	P2Y14
BB3	GPRC5D	N-formylpeptide	Tachykinin
Cannabinoid	Histamine	FPR1	NK2
CB2	H2	Neuropeptide Y	Vasopressin/Oxytocin
GPR55	Leukotriene/Lipoxin	Y1	V2
Chemokine	ALX	Neuromedin U	Orphan
CXCR1	BLT1	NMU1	GPR18
CXCR3	BLT2	Nicotinic Acid	GPR35
CXCR4	CysLT1	GPR109A	GPR84
CCR1	Lysophospholipid	PAF	GPR92
CCR5	LPA1	PAF	GPR101
CCR9	LPA2	Prostanoid	GPR171/H963
Corticortropin-releasing	LPA3	GPR44	MAS1
CRF1	S1P1	FP	MRGF
Free Fatty Acid	S1P2	Protease-activated	PSGR
FFA1	S1P3	PAR2
FFA2	S1P4	PTH
FFA3	S1P5	PTH2

Case Study : Identification of Multiple Cross-Reactivities of Anti-CCR5

The N-term-tagged GPCRs are expressed in HEK293T cells. Binding of a test mAb to the GPCR receptor panel is measured by FACS analysis using Geometric Mean of fluorescence intensity. RBI (Relative Binding Index) is determined by normalizing Geometric Mean of the test mAb binding to that of anti-tag mAb binding. RBI for the test mAb to the target GPCR is set at 100%.