GPCR Monoclonal Antibody Profiling
Issue: There are 350 "druggable" GPCRs which share many similar primary and conformational epitopes. Is your antibody lead SPECIFIC to your GPCR target of interest?
Solution: Assess specificity of the antibody lead in Multispan's large GPCR expression panel. We measure your antibody binding to GPCRs in NATIVE conformations directly on mammalian cell surface by FACS.
Benefits: Obtain valuable information to
-make Go-Or-No-Go decision early
-save cost in expensive animal and clinical studies
-shorten lead development cycle
Convenience: Send us your purified, un-labeled antibody and you will have the results back in 1 week.
Flexibility: Choose your own panel from our 320 human and 180 murine full-length cDNAs in Multispan's powerful proprietary expression vectors.
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Case Study : Identification of Multiple Cross-Reactivities of Anti-CCR5
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| The N-term-tagged GPCRs are expressed in HEK293T cells. Binding of a test mAb to the GPCR receptor panel is measured by FACS analysis using Geometric Mean of fluorescence intensity. RBI (Relative Binding Index) is determined by normalizing Geometric Mean of the test mAb binding to that of anti-tag mAb binding. RBI for the test mAb to the target GPCR is set at 100%.
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