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Eblast 3 March 2023 Title-4

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Need to screen for antibody internalization in high throughput?

 

We have the assay for you!

• Simplicity: live cell flow cytometry (FACS)

 

• Small epitope-tag: control for target expression

 

• Adaptable for HTS: 96-well format

 

• Expression-based: applicable to orphan GPCRs!

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Binding Non-Specificity of Anti-CCR5 Monoclonal Antibody

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GPCR Cell-signaling and Internalization

 

β-arrestin recruitment promotes receptor internalization via clathrin-coated pits; Some GPCRs are internalized via arrestin-independent mechanisms

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