MULTISCREEN™ β-Arrestin Assay Kits

No-wash homogeneous luminescence assays for intracellular β-Arrestin translocation detection in 96-well, 384-well and higher multiplexity. Our proprietary and patented MULTISCREEN™ Beta-Arrestin recruitment assay technology overcomes the receptor-tagging drawback of other technologies, enabling high throughput detection of beta-arrestin translocation induced by native GPCRs in vitro and in vivo.
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Why MULTISCREEN™ β-Arrestin?

Product Features
  • Native, unmodified GPCRs. No receptor tagging required, unlike other β-arrestin technologies.
  • Proprietary, patented sensor technology. Multispan-developed β-arrestin translocation detection.
  • In vitro and in vivo capable. First technology enabling both in high-throughput format.
  • No-wash, homogeneous luminescence. Minimal hands-on time, designed for HTS.
  • HTS-ready. Validated in 96-, 384-, and higher-density formats.
  • Three kit configurations. Standard, BacMam, and HSV viral delivery.
  • Sub-30 minute hands-on time.
  • Cross-validated with MULTISCREEN™ stable cell lines and division-arrested cells.

Available Assay Kits

Unit Size (384-Well) Catalog Number
1 plate 96-well/384-well
MSBAK01-1
10 plates 96-well/384-well
MSBAK01-10
100 plates 96-well/384-well
MSBAK01-100
Contains the substrate for MULTISCREEN™ β-Arrestin sensors and assay buffer. Validated by MULTISCREEN™ stable cell lines co-expressing target GPCRs and MULTISCREEN™ β-Arrestin sensors, and by MULTISCREEN™ GPCR stable cell lines and division-arrested cells. Suitable for any cell line or primary cells co-expressing endogenous or untagged GPCR target and MULTISCREEN™ β-Arrestin sensor.
β-Arrestin Recruitment Dose Response in MULTISCREEN™ MOR β-Arrestin2 CHO-K1 stable cell line (CA1350-1a) measured with MULTISCREEN™ β-Arrestin Assay Kit. Cells were seeded in assay buffer in a 384-well plate, incubated with the MULTISCREEN™ Substrate before being treated with control agonist DAMGO and measured by Flexstation 3.
Unit Size (384-Well) Catalog Number
1 plate 96-well/384-well
β-Arrestin1 MSBAKBM01-1 Or β-Arrestin2 MSBAKBM02-1
10 plates 96-well/384-well
β-Arrestin1 MSBAKBM01-10 Or β-Arrestin2 MSBAKBM02-10
100 plates 96-well/384-well
β-Arrestin1 MSBAKBM01-100 Or β-Arrestin2 MSBAKBM02-100
Viral delivery kit containing BacMam viral particles of MULTISCREEN™ β-Arrestin sensor cDNA, control GFP BacMam viral particle, the substrate, and assay buffer. Validated by MULTISCREEN™ GPCR stable cell lines and division-arrested cells. Suitable for any cell line or primary cells expressing endogenous or untagged GPCR target.
β-Arrestin Recruitment Dose Response in MULTISCREEN™ MOR CHO-K1 stable cell line (C1350-1a) infected with β-Arrestin sensor BacMam virus for 48 hours before being measured with MULTISCREEN™ β-Arrestin Assay Kit. Cells were seeded in a 384-well plate, incubated with the MULTISCREEN™ Substrate before being treated with control agonist DAMGO and measured by Flexstation 3.
Unit Size (384-Well) Catalog Number
1 plate 96-well/384-well
β-Arrestin1 MSBAKHSV01-1 Or β-Arrestin2 MSBAKHSV02-1
10 plates 96-well/384-well
β-Arrestin1 MSBAKHSV01-10 Or β-Arrestin2 MSBAKHSV02-10
100 plates 96-well/384-well
β-Arrestin1 MSBAKHSV01-100 Or β-Arrestin2 MSBAKHSV02-100
Viral delivery kit containing Herpes Simplex viral (HSV) particles of MULTISCREEN™ β-Arrestin sensor cDNA, control GFP HSV viral particle, the substrate, and assay buffer. Validated by MULTISCREEN™ GPCR stable cell lines and division-arrested cells. Suitable for any cell line or primary cells expressing endogenous or untagged GPCR target.
β-Arrestin Recruitment Dose Response in MULTISCREEN™ KOR HEK293T stable cell line (C1352) infected with β-Arrestin HSV sensor for 48 hours before being measured with MULTISCREEN™ β-Arrestin Assay Kit. Cells were seeded in a 384-well plate, incubated with the MULTISCREEN™ Substrate before being treated with control agonist Dynorphin B and measured by Flexstation 3.

Citations and Publications

Identification and In Vivo Evaluation of Myelination Agent PIPE-3297, a Selective Kappa Opioid Receptor Agonist Devoid of β-Arrestin-2 Recruitment Efficacy

GPCR Target: Membranes isolated from CHO-K1 cells stably expressing human KORs (cat. no. MC1351-1a), MULTISCREENᵀᴹ β-arrestin-2 recruitment assay

Authors: Schrader, T. O., et al.

Journal: ACS Chemical Neuroscience, 15(3), 685–698. 

Year Published: 2024

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